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Day Laboratory

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Dr. Brian Day’s laboratory is located on the 4th floor of the Smith building on National Jewish Health’s campus.

Research Methods

Quantitating Oxidants

  • Superoxide
  • Hydrogen Peroxide
  • Hypohalous Acids

 

Quantitating markers of oxidative stress

  • Lipid oxidation
  • 4HNE by GC/MS
  • F2 isoprostane by GC/MS
  • 7-OH-cholesterol by GC/MS
  • Protein oxidation
  • Nitro tyrosine by HPLC-EC
  • Chloro-tyrosine by HPLC-EC
  • Protein- GSH by HPLC-EC
  • GSH/GSSG
    • Spectrophotometric
    • HPLC-FD
    • HPLC-EC
  • DNA oxidation
  • 8-OH-2dG by HPLC-EC

 

Pharmacokinetics of drugs and biomarkers (non-GLP)

  • HPLC

 

Pharmaceutical Formulations and Delivery Systems

  • Extended release sub cutaneous injection
  • Inhalation delivery systems

 

Therapeutic Safety Assessments (non-GLP)

  • Ames Assay
  • Maximum Tolerated Dose
  • NOEL
  • Therapeutic Index

 

Animal Models

  • SOD1 KO
  • SOD2 KO
  • CFTR KO
  • GCLM KO
  • bENaC Tg

 

Human CF Bacterial Isolates

  • Pseudomonas aeruginosa
  • Burkholderia cepacia complex
  • Staphylococcus aureus
  • Mycobacterium abscesses

 

Patents

  1. Oxidant Scavengers, US #5,994,339 (1999)
  2. Oxidant Scavengers, US #6,127,356 (2000)
  3. Paraquat, Dipyridinium-Based Inhibitors of Nitric Oxide Synthase, Related Compounds & Therapeutic Uses Thereof, US #6,231,894 (2001); Australian #778,967
  4. Substituted Porphyrins, US #6,479,477 (2002)
  5. Substituted Porphyrins, US #6,544,975 (2003)
  6. Oxidant Scavengers, US #6,583,132 (2003)
  7. Methods for Identifying Compounds as Antioxidants, US #6,900,026 (2005)
  8. Substituted Porphyrins, US #6,916,799 (2005)
  9. Substituted Porphyrins, US #7,189,707 (2007)
  10. Oxidant Scavengers, US #7,470,677 (2008)
  11. Methods for treatment of thiol-containing compound deficient conditions, US #7,498,047 (2009)
  12. Compounds and methods for thiol-containing compound efflux and cancer treatment, US#7,790,762 (2010)
  13. Substituted Porphyrins, US#7,820,644 (2010)
  14. Substituted Porphyrins, US#8,217,026 (2012)
  15. Oxidant scavengers for treatment of type I or type II diabetes, US#8,470,808 (2013)
  16. Substituted Porphyrins, US#8,546,562 (2013)
  17. Cancer Therapy, US#8,765,729 (2014)
  18. Substituted Porphyrins, US#8,946,202 (2015)
  19. Substituted Porphyrins, US#9,289,434 (2016)
  20. Methods and compositions for treating infection and inflammation with selenocyanate, US #10,751,315 (2020)